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Diagnostics

MVP's Diagnostic Laboratory has provided swine and bovine veterinarians and their clients with reliable, accurate, and timely diagnostic services since 1984. With state-of-the-art facilities, MVP is one of the few laboratories in the U.S. that can successfully isolate all species of Mycoplasmas.

MVP has developed specialized media and isolation techniques for M. hyopneumoniae, M. hyosynoviae (patent pending), M. hyorrhinis and M. bovis. We utilize PCR protocols to speciate these mycoplasmas and to detect the presence of specific critical genes known as "virulence factors."

In addition, MVP has implemented assays to quantify the production of alpha toxin and beta 2 toxin from any given Clostridium perfringens isolate. This technology assures our clients that the vaccine produced actually contains the protective antigens as many isolates do not carry these virulence genes or express them.

MVP has developed proprietary technology for isolation and growth of Cl. difficile, an organism that is exceedingly difficult to grow to an acceptable antigen mass. MVP's Cl. difficile vaccines have been very successful in the field.

MVP has created immunohistochemistry staining techniques for the detection of microorganisms in tissue and the use of Polymerase Chain Reaction (PCR) to identify, characterize, and genotype microorganisms.

MVP pioneered the characterization of Actinobacillus pleuropneumoniae and Streptococcus suis through serotyping.

Molecular Biology

  • Actinobacillus pleuropneumoniae identification and genotyping
  • Haemophilus parasuis identification and subtyping
  • E. coli pilus F4, F5, F6, F18, F41
  • E. coli toxins STa, STb, LT, VT2e, EAST1
  • E. coli adhesin AIDA-I, paa
  • Clostridium perfringens toxin Types A, B, C, D, E, Beta 2, ET
  • Clostridium difficile toxin A and B
  • Mycoplasma speciation for Bovine, Canine, Porcine species
  • Mycoplasma hyopneumoniae adhesin gene detection
  • Pasteurella multocida toxA, dermonecrotic toxin gene
  • Erysipelas rhusiopathiae identification and DNA fingerprinting
  • Camphylobactor jejuni and coli identification
  • Swine Influenza Virus identification
  • PRRS identification NA and EU strain

Additional tests available, call for further information.

Bacteriology

Bacterial culture, isolation, and identification.

We accept cultures from state and commercial laboratories and from clinics for confirmation of identification, characterization, and autogenous bacterin production.

Antibiotic susceptibility testing on aerobic bacteria.

Serotyping:

  • Actinobacillus pleuropneumoniae serotypes 1-12
  • Streptocccus suis serotypes 1-34
  • Haemophilus parasuis 7 serotypes and genotyping, PCR
  • Pasteurella A and D typing
  • Salmonella species O Grouping

Virology 

  • Viral isolation, identification
  • SIV
  • PRRS
  • PCV2

Immunohistochemistry

  • SIV (H1N1 and H3N2) strains
  • PRRS US strain
  • PCV2
  • BVD ear notch (out sourced)

Serology

  • Actinobacillus pleuropneumoniae
  • Haemophilus parasuis
  • Mycoplasma bovis
  • Mycoplasma hyopneumoniae
  • PRRS

Autogenous Isolate Selection Services

  • Protein electrophoresis antigen assay
  • Clostridium perfringens alpha toxin (phospholipase C) production
  • Quantitative assay

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